Production of Membrane Proteins: Strategies for Expression by Anne Skaja Robinson

By Anne Skaja Robinson

Designed as a research-level consultant to present options and techniques of membrane protein creation at the small to intermediate scale, this practice-oriented publication offers certain, step by step laboratory protocols in addition to an evidence of the rules at the back of each one strategy, including a dialogue of its relative benefits and drawbacks.
Following an introductory part on present demanding situations in membrane protein creation, the ebook is going directly to examine expression structures, rising tools and ways, and protein particular considerations.
Case experiences illustrate the right way to pick out or pattern the optimum construction approach for any wanted membrane protein, saving either money and time at the laboratory in addition to the technical creation scale.
designated in its insurance of "difficult" proteins with huge membrane-embedded domain names, proteins from extremophiles, peripheral membrane proteins, and protein fragments.

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